Figure 4

Proximity of substitution mutations to β-mannosidase active site. Amino acids are colored in red, magenta and yellow corresponding to the catalytic nucleophiles, ligand binding site residues and residues involved in substitutions, respectively. The active site residues and the residues involved in substutions are shown as sticks, while the rest of the protein is in ribbon representation. The model shown is that of WT human β-mannosidase.