Figure 5

Phylogenetic analysis of full-length CYP450s from P. notoginseng and characterized CYP450s from other plants. Amino acid sequences were aligned using the CLUSTALW program, and evolutionary distances were computed using MEGA4 with the Poisson correction method. Bootstrap values obtained after 1000 replications are indicated on the branches. Values less than 50% are not shown. The GenBank/EMBL/DDBJ accession numbers of the sequences are: U48434 (Solanum chacoense), O81971 (Glycine max), AJ238612 (Catharanthus roseus), AF124816 (Mentha × piperita), Z17369 (Helianthus tuberosus), Q42797 (Glycine max), D14588 (Petunia × hybrida), AB035868 (Arabidopsis thaliana), U54770 (Solanum lycopersicum), AB433175 (Medicago truncatula), AB433176 (Medicago truncatula), AB433177 (Lotus japonicus), AB433178 (Lotus japonicus), AB433179 (Glycyrrhiza uralensis), AF537321 (Pisum sativum), AF537322 (Pisum sativum), AF318500 (Arabidopsis thaliana), AF318501 (Arabidopsis thaliana), AF326277 (Hordeum vulgare), AF135485 (Glycine max), AB231332 (Glycine max), AB023636 (Glycyrrhiza echinata), AB437320 (Glycyrrhiza uralensis), D83968 (Glycine max), X71657 (Solanum melongena), X71658 (Solanum melongena), X71656 (Solanum melongena), X71655 (Solanum melongena), L23209 (Zea mays), NM_001112599 (Zea mays), U29333 (Pisum sativum), U69134 (Arabidopsis thaliana), U18929 (Arabidopsis thaliana), AF150881 (Solanum lycopersicum × Solanum peruvianum), AF214008 (Brassica napus), U38416 (Arabidopsis thaliana), AF029856 (Sorghum bicolor), AF029858 (Sorghum bicolor), AJ583531 (Triticum aestivum).