A plot of genome coverage against normalized average depth. Duplicate data sets were normalized and pooled. Variance in coverage above and below the normalized average depth (red vertical line) across the genome is shown. Deviation of sample curves from the average depth indicates level of evenness in coverage depth distribution across the genome. The closer the sample curve is to the vertical line, the more even the coverage. The theoretical curve represents average normalized depth at 100% genome coverage. A) Coverage by libraries made from P. falciparum 3D7 (1 normalized depth represents 21×). B) Coverage by libraries made from clinical isolate, PK0076 (1 normalized depth represents 11×). Kapa HiFi, Kapa2G and Platinum pfx enzymes were used in the presence of TMAC.