Structure and sequence conservation of P. chabaudi AAT1 and PCHAS_031370 and their orthologues. Secondary structure predictions of AAT1 (A) and PCHAS_031370 (C) proteins reveal 10 and 12 TM-helix proteins, respectively. The mutations discovered within CQ-R and CQ-hiR P. chabaudi parasites (AS-3CQ and AS-30CQ, respectively) are highlighted (magenta). The alignments of Plasmodium spp. protein fragments (B, D) indicate the positions of mutations in, or close to, conserved regions.