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Table 1 Phenotype and Genotype of Selected Clones of the AS P.chabaudi Lineage

From: Quantitative genome re-sequencing defines multiple mutations conferring chloroquine resistance in rodent malaria

   gene abbreviation, chromosome, residue
   dhfr 1   aat1 1 0313702 cir 1 ubp1 1 1015502    
   chr07   chr11 chr03 chr03 chr02 chr10 chr14 chr07 chr05
   106   173 102 719 707 2728 162 intergenic intergenic  
Clone or Strain PYR response   CQ response          
AJ Sensitive S Sensitive A I T T V Y wt wt wt
AS-sens Sensitive S Sensitive A I T T V Y wt wt wt
AS-PYR Resistant N Sensitive A I T T V Y mut 34 bp deletion wt
AS-3CQ Resistant N Resistant E I T T V Y mut 34 bp deletion > 1 kbp deletion
AS-15CQ this line is uncloned 3 3
AS30CQ Resistant N Hi-Resistant E I N N F H mut 34 bp deletion > 1 kbp deletion
AS-15MF4 Resistant N Resistant E del4 T T F Y mut 34 bp deletion > 1 kbp deletion
  1. The pyrimethamine (PYR) and CQ responses of clones of the AS lineage are shown along with all mutations detected in AS-30CQ (and other clones) relative to AS-sens. 1 dhfr, dihydrofolate reductase; aat, aminoacid transporter; cir, chabaudi interspersed repeat; ubp, ubiquitin specific protease (de-ubiquitinating enzyme). 2 Refers to genes PCHAS_031370 or PCHAS_101550. 3 mixed genotype and phenotype, see Additional File 1. 4 Note that AS-15MF carries an alternative allele of PCHAS_031370, namely I102del. Single-letter amino acid code used: A, alanine; E, glutamate; F, phenylalanine; H, histidine; I, isoleucine; N, asparagine; S, serine; T, threonine; V, valine; Y, tyrosine; del, codon deleted.