Table 5 Primers used for the validation of gene expression and alternative splicing
Gene | Primer sequence 5'→3' | Amplicon size (bp) |
|---|---|---|
Primers for gene expression validation | ||
GBG5 | F: TCCAGCGTCGCGGCTATGAA | 94 |
R: TCAAATCTGCAGCTGCCTGGGA | ||
PGP | F: TGAAGCGGCTGGGCTTCCCTAT | 131 |
R: GGGCACCAACATGGACAACCGA | ||
QOP5F6 | F: TGGTGACGTGCACCCGAATGA | 112 |
R: AGCACCCACGATGGGGAAGATC | ||
CFL1 | F: TGGTGTCGACGACTTACGCACT | 132 |
R: AGCGCCTCTCGTCTTGTAGGCT | ||
B4GALT4 | F: TGATACCGGCCTCTGTGCACCT | 113 |
R: TGCCCTTCTGTGTCTCCACACCT | ||
MYL7 | F: ACCCCAGTGGTAAAGGCGTGGT | 115 |
R: TCCATGGGTGTCAGGGCGAACA | ||
DUS6 | F: GGGCGAACTCGGCTTGGAACTT | 118 |
R: AACGAGAATACGGGCGGCGA | ||
Primers for alternative splicing validation | ||
AP2B1-F1 | GCCTAGATAGTCTGCTTGGCA | 134 |
AP2B1-R | ACCACTGCTGACCACAGC | |
AP2B1-F2 | GCCTAGATAGTCTGGTGGGA | 92 |
AP2B1-R | ACCACTGCTGACCACAGC | |
ZDHHC16-F1 | TGCTGCCATCGAGACTTA | 97 |
ZDHHC16-R | GCACAGGAACCAGAGGTAGAC | |
ZDHHC16-F2 | CGACAAGAACAAACTACAGG | 118 |
ZDHHC16-R | GCACAGGAACCAGAGGTAGAC | |