Validation of differentially expressed genes at the RNA and protein levels in HeLa cells. Differential expression of up- ( A) or downregulated ( B) genes in HeLa cells transfected with pCAGGS encoding Flag-tagged Tax proteins (TaxWT, open column; TaxS240, shaded column; TaxD247G, black column) or the control pCAGGS (light column) was validated by real-time RT-PCR. The bars indicate the fold change expression of the genes regulated by Tax. Data were normalized to GAPDH mRNA expression. The results represent the mean of 2 samples from 1 experiment. (C) Protein expressions of Tax, Fos, Rora, Rrad, Gem, and Tnfaip6 were validated by western blotting. Actin was used as a control.