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Figure 4 | BMC Genomics

Figure 4

From: Identification of bovine leukemia virus tax function associated with host cell transcription, signaling, stress response and immune response pathway by microarray-based gene expression analysis

Figure 4

Validation of differentially expressed genes at the RNA and protein levels in HeLa cells. Differential expression of up- ( A) or downregulated ( B) genes in HeLa cells transfected with pCAGGS encoding Flag-tagged Tax proteins (TaxWT, open column; TaxS240, shaded column; TaxD247G, black column) or the control pCAGGS (light column) was validated by real-time RT-PCR. The bars indicate the fold change expression of the genes regulated by Tax. Data were normalized to GAPDH mRNA expression. The results represent the mean of 2 samples from 1 experiment. (C) Protein expressions of Tax, Fos, Rora, Rrad, Gem, and Tnfaip6 were validated by western blotting. Actin was used as a control.

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BMC Genomics

ISSN: 1471-2164

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