Figure 1

Secretion of cellulolytic enzymes by wild type, Δ wc-1 , Δ wc-2 and Δ vvd mutants. Azo- CMC cellulase (endoglucanase) activity in culture filtrates of N. crassa wild type (WT) and mutant strains upon growth in Birds-medium with 2% (w/v) Avicel cellulose as sole carbon source at 16, 28 and 40 hrs versus 5 days. The flasks show examples for degradation of Avicel in wild type (left) and Δvvd mutant (right) strains after 40 hours of growth. At this timepoint the cellulosic substrate was almost or completely degraded by the strains used in this study. Error bars show standard deviation of at least three biological replicates.