Gene expression analysis of Drosophilaa Manf mutants reveals perturbations in membrane traffic and major metabolic changes

Table 1 Overview of microarray experiment

comparison between genotypes	diff. reg. probes	% all probes	down reg.	% all diff. reg.	upreg.	% all diff. reg.
*Manf* ^mz ^Δ ⁹⁶ vs wt stage 17 embryos	3183	7.3	1501	47.2	1682	52.8
*Manf* ^mz ^Δ 96 / + vs wt stage 17 embryos	180	0.4	53	29.4	126	70.0
*Manf* ^mz ^Δ96 vs *Manf* ^mz ^Δ ⁹⁶ /+ stage 17 embryos	2681	6.2	1290	48.1	1391	51.9
*Manf* ^Δ96 vs *wt 29* -50 hr AEL larvae	1734	4.0	894	51.6	840	48.4
*69B* -GAL4> *UAS-Manf* ¹³³ vs wt 29-50 hr AEL larvae	1240	2.8	513	41.4	727	58.6
*Manf* ^Δ96 vs *69B* -GAL4 > UAS- *Manf* ¹³³ 29-50 hr AEL	2775	6.4	1615	58.2	1160	41.8

The number of differentially regulated probes was compared. In the Agilent Drosophila microarray design (4 × 44 K) there was unequal number of probes targeting the particular gene, ranging from one probe to several per gene.

ISSN: 1471-2164