Figure 4

Autoradiograph of TD gel of mGing in seedlings germinated from seeds after irradiation. The gDNA samples were digested with a hexacutter restriction enzyme PstI and ligated to the PstI cassettes for TD analysis. Seeds collected from a single inbred spike of japonica Jiahua No. 1 were divided into three groups and received γ-ray irradiation at different doses before germination. Group I, gDNAs from tiller leaves of 14 plantlets geminated from seeds with no irradiation; Group II, gDNAs from tiller leaves of 22 plantlets germinated from seeds with γ-ray irradiation at 300 Gy; Group III, gDNAs from tiller leaves of 10 plantlets germinated from seeds with γ-ray irradiation at 500 Gy. A rice MITE, ID-1, was used as control for group III. Lanes with asterisk showed the gDNA samples of irradiated seedlings were selected for the confirmation of mGing transposition by reverse PCR.