Figure 5

PCR validation of mGing active transposition in γ-ray irradiation Experiment. (a) Agarose gel analyses of PCR products of mGing transpositions at three loci. Two bands of different molecular weight were identified in an insertion locus (R5) and two excision loci (R9, R13) from ten irradiated-seedlings (lane 1 to 10). Clone D was used as a control. The amplicons were indicated as the higher molecular weight band (HW) or the lower molecular weight band (LW). (b) Sequence alignment of three transposition loci. Each sequence name corresponded to that in (a). A plus mark used as a superscript to indicate sequences contained an mGing element. Internal sequences of mGing elements were indicated as black arrows, while the TIR sequences of mGing elements were shaded. The SNPs were boxed. The dashed line represents gap, and the brackets with three dots indicates the missing sequences.