PCR validation of mGing active transposition in anther culture experiment. (a) Agarose gel analyses of PCR products of mGing transposition loci in anther culture experiment. Two bands of different molecular weight were identified in two new transposition loci (A4 and A6) from 14 plantlets regenerated from anther-derived calli (lane 1 to 14). (b) Sequence alignment of two transposition loci. Each sequence name corresponded to that in (a). Clone D was used as a control in both experiments. The amplicons were indicated as the higher molecular weight band (HW) or the lower molecular weight band (LW). A plus mark was used to identify the sequence with an mGing element. The dashed line represents gap and the brackets with three dots indicates the missing sequences. The TIRs of mGing elements were shaded, the TSDs were underlined, and the SNPs were boxed.