A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica

Table 1 Sequencing, masking and trimming statistics for unigene assembly

	Sequencing method
	Sanger							Pyrosequencing
Library	CMFL	CFFL	CLFL	CM	CF	CC	Total	TUM25
Number of reads (1)	39936	N/A	N/A	3456	768	5184	129195	1333444
Number of 3' ESTs in (1)	19968	N/A	N/A	2880	0	0	62776	-
Number of base call (amount of sequences in Mbp) for (1)	30.12	N/A	N/A	2.97	0.53	4.95	141.66	393.8
Amount of bases (Mbp) masked after cross_match	4.07	N/A	N/A	0.16	0.16	0.59	14.04	0.09
Number of reads after SeqClean (reads passed to assembly) (2)	36722	N/A	N/A	3175	485	4435	118319	1201150
Amount of reads (Mbp) after SeqClean (reads passed to assembly)	19.95	N/A	N/A	1.96	0.23	2.26	78.59	354.5
Aveage read length (bp) in (2) with phred QV > =20	535.8	N/A	N/A	609.3	460.2	500.5	654.2	282.7
Tissue/developmental stage	Male bud	Female bud	Leaf	Male flower	Female flower	Inner bark	–	Seedling
References	[17]	Futamura et al. in prep.	Futamura et al. in prep.	[19]	[19]	[20]	–	This study

ISSN: 1471-2164