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Table 1 Sequencing, masking and trimming statistics for unigene assembly

From: A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica

  Sequencing method
  Sanger Pyrosequencing
Library CMFL CFFL CLFL CM CF CC Total TUM25
Number of reads (1) 39936 N/A N/A 3456 768 5184 129195 1333444
Number of 3' ESTs in (1) 19968 N/A N/A 2880 0 0 62776 -
Number of base call (amount of sequences in Mbp) for (1) 30.12 N/A N/A 2.97 0.53 4.95 141.66 393.8
Amount of bases (Mbp) masked after cross_match 4.07 N/A N/A 0.16 0.16 0.59 14.04 0.09
Number of reads after SeqClean (reads passed to assembly) (2) 36722 N/A N/A 3175 485 4435 118319 1201150
Amount of reads (Mbp) after SeqClean (reads passed to assembly) 19.95 N/A N/A 1.96 0.23 2.26 78.59 354.5
Aveage read length (bp) in (2) with phred QV > =20 535.8 N/A N/A 609.3 460.2 500.5 654.2 282.7
Tissue/developmental stage Male bud Female bud Leaf Male flower Female flower Inner bark Seedling
References [17] Futamura et al. in prep. Futamura et al. in prep. [19] [19] [20] This study
  1. N/A: See Futamura et al. in preparation for details.