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Table 1 Sequencing, masking and trimming statistics for unigene assembly

From: A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica

 

Sequencing method

 

Sanger

Pyrosequencing

Library

CMFL

CFFL

CLFL

CM

CF

CC

Total

TUM25

Number of reads (1)

39936

N/A

N/A

3456

768

5184

129195

1333444

Number of 3' ESTs in (1)

19968

N/A

N/A

2880

0

0

62776

-

Number of base call (amount of sequences in Mbp) for (1)

30.12

N/A

N/A

2.97

0.53

4.95

141.66

393.8

Amount of bases (Mbp) masked after cross_match

4.07

N/A

N/A

0.16

0.16

0.59

14.04

0.09

Number of reads after SeqClean (reads passed to assembly) (2)

36722

N/A

N/A

3175

485

4435

118319

1201150

Amount of reads (Mbp) after SeqClean (reads passed to assembly)

19.95

N/A

N/A

1.96

0.23

2.26

78.59

354.5

Aveage read length (bp) in (2) with phred QV > =20

535.8

N/A

N/A

609.3

460.2

500.5

654.2

282.7

Tissue/developmental stage

Male bud

Female bud

Leaf

Male flower

Female flower

Inner bark

Seedling

References

[17]

Futamura et al. in prep.

Futamura et al. in prep.

[19]

[19]

[20]

This study

  1. N/A: See Futamura et al. in preparation for details.