Figure 5

Prominent genetic features of plasmid pJA144188 from C. resistens DSM 45100 (A), Genetic structure of corynebacterial class 1 integrons. A comparison of the class 1 integron from pJA144188 with those present on plasmids pTET3 and pCG4 from C. glutamicum strains is shown. The filled circles indicate the position of 59-bp elements downstream of gene cassettes. The aacA1 and gcuG genes represent a gene pair that is not separated by a 59-bp element. The cassette integration site attI1 and the terminal repeat sequences (TR) are shown as black boxes. Deletions characterizing the class 1 integrons on pJA144188 and pTET3 are indicated. (B), Detailed view of the tetracycline resistance gene region of pJA144188. The tet(W) gene region is presented including the predicted leader peptide gene tetLP. The truncated insertion sequences ISCx1 and IS26 flanking the tet(W) gene region are shown as yellow boxes. These remnants of insertion sequences form the boundaries of a 2,323 bp DNA sequence that is also present in Streptococcus suis, Arcanobacterium pyogenes, and Lactobacillus reuteri, with the exception of three terminal nucleotides at the 5' end.