Figure 5

Murine infection studies with three different Listeria serotypes and two chromosomal deletion mutants of ∆ lmaB and ∆ lmaD of L. monocytogenes 1/2a EGD-e Mice were infected i.v. with 2000 cfu of L. monocytogenes serotypes 1/2a EGD-e (filled circles), 4b F2365 (open circles), 4b CLIP80459 (filled triangles), and 4a L99 (open triangles). On days 1, 3, 5, and 8 after infection, the numbers of viable bacteria in spleens (A) and livers (B) of three animals per group were determined (P ≤0,05 and P ≤0,001 of 4b CLIP80459 vs. 1/2a EGD-e and 4b F2365 vs. 1/2a EGD-e in spleen and liver respectively). Bacterial load in mice organs were also determined following i.v. infection with 2000 cfu of L. monocytogenes 1/2a EGD-e wild type strain (filled circles) as well as its isogenic mutants ∆lmaB (open circles), and ∆lmaD (filled triangles). On days 1, 3, and 5 after infection, the numbers of viable bacteria in spleens (C) and livers (D) of three animals per group were determined (P ≤0,05 and P ≤0,01 of 1/2a EGD-e versus ∆lmaB and ∆lmaD in spleen and liver respectively). Data presented are representative of three independent experiments. An asterisk indicates means that are significantly different from the wild type. Significance analysis was performed with student t-test.