Table 7 Primers used for experimental validation of intron positions
From: The Physalis peruviana leaf transcriptome: assembly, annotation and gene model prediction
COSII Marker | Primer sequences (5′ – 3′) | P. peruvianaunique identifier | Primer position in the cDNA |
|---|---|---|---|
C2_At3g07100 | ACGAACGATGTGCTGCTGGATATAC | Php00a01046.06900 | F-1156/1178 |
AGACCCTGGGGATCTAAGCTCTCTG | R-997/1022 | ||
C2_At 2 g35920 | TGCTTGCAACCAACATAGCTGAG | Php00a05845.15798 | F-424/446 |
AAGCTCTTGTAGTGGTGTTCGAAG | R-172/195 | ||
C2_At 3 g06580 | TGCTCAACTCACATGTGAGTGTGAAAG | Php00a06435.16388 | F-715/740 |
AGCAAACCCAGATTTTGCCATAAC | R-784/806 | ||
C2_At 5 g41480 | TATTCGTGCTGGTCTGGAGAGTGC | Php00a02812.11168 | F-836/859 |
ATGATCCTTGTCATTCGCCATAGC | R-646/669 | ||
C2_At 5 g27620 | ATCTACAATGGTCCGTGATGGAAC | Php00a03329.12202 | F-776/799 |
TTCCTCTGCCTTGCAAGCTGC | R-720/740 | ||
C2_At 3 g04870 | ACGCGTGCTAGTATCCAGAGG | Php00a02563.10671 | F-1226/1246 |
TGACATGGCAAAGCCCACTAACATAC | R-954/977 | ||
C2_At 5 g60160 | ACACAATGCTAATCAACGTTATGC | Php00a01985.09526 | F-278/297 |
TCATCCACCGCGCACATTTC | R-482/505 |