Evolution of vertebrate interferon inducible transmembrane proteins

BMC Genomics

Table 3 Primers and RT-PCR conditions used to amplify tammar IFITMs

Gene	Forward	Reverse	Number of PCR cycles	Annealing temperature
IFITMA1	GTGCTCTGCGCTCCCTGT	AGCCATTCCCCAACAAATA	35	58*
IFITMA2	GGCTCGCAGTGCTACAGTCT	AAGTGAAGAGAGGCCACACC	30	60*
IFITMB	AGGAGCGCTATCCTGTGTGT	CTAGGGAAGCATGCAGGTGT	35	59*
IFITM10	CCTTCAGCAGCCTCCTCAG	AGTTCAGGTCAGTGGGATGG	40	58*
IFITM5	GACCTATGCGGCGAGTCCGC	GAAAGGAAGGAGGGCATCTT	40	58*
GAPDH	CCTACTCCCAATGTATCTGTTGTGG	GGTGGAACTCCTTTTTTGACTGG	30	60

ISSN: 1471-2164