Table 1 Summary of individual components of the insertion vector
DNA sequence | Function |
|---|---|
ATG-less λ buffer | Protect the lexA-HSP70 promoter and the barcode from degradation |
HSP70 promoter | Drive the expression of adjacent genes |
lexA site | The binding site for LexA-VP16 protein for inducible activation of the HSP70 promoter |
Random barcode | A specific DNA tag in each mutant for tracking mutant frequency |
ura4+ ORF/DNA | The selectable marker |