Mutation | Phenotype | AmiGO or KEGG expectation | Expected number in 3581 mutants | Actual number of isolates |
---|---|---|---|---|
Auxotrophy | Slow or no growth on minimal medium | 2.4% (94a/5122b) | 37c | 30 |
Defective adenine biosynthesis | Colony color change from pale pink to white or red on low adenine medium | NDd | NDd | 13 (whited) |
0.04% (2e/5122 b) | 1-2e | 1 (red) | ||
Temperature sensitivity | Slow or no growth at 36°C | NDf | NDf | 25 |
EtBr resistance (petite positivity) | Growth in medium with EtBr | NDf | NDf | 13 |