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Table 2 Assessment of mutation diversity in the barcode-tagged insertion mutant library by four genetic screens

From: Generation and analysis of a barcode-tagged insertion mutant library in the fission yeast Schizosaccharomyces pombe

Mutation

Phenotype

AmiGO or

KEGG expectation

Expected number in 3581 mutants

Actual number of isolates

Auxotrophy

Slow or no growth on minimal medium

2.4% (94a/5122b)

37c

30

Defective adenine biosynthesis

Colony color change from pale pink to white or red on low adenine medium

NDd

NDd

13 (whited)

0.04%

(2e/5122 b)

1-2e

1 (red)

Temperature sensitivity

Slow or no growth at 36°C

NDf

NDf

25

EtBr resistance (petite positivity)

Growth in medium with EtBr

NDf

NDf

13

  1. a. The estimated number is from AmiGO database [33] by searching genes using keywords “amino acid biosynthesis” and “nucleobase biosynthesis”, and excluding genes involved in the adenine, histidine, leucine and uracil pathways as the parental strain in defective in these pathways and all mutants are Ura+.
  2. b. Total gene number in S. pombe genome (as of 2/27/2012) = 5122.
  3. c. The estimated number is calculated as “the total number of mutants tested (3581)” × “AmiGO expectation” × “the fraction of protein coding sequences in the total S. pombe genome (57%)”.
  4. d. Not determinable; white colony color could also result from mitochondrial defects [34, 35].
  5. e. The estimated number is from KEGG [36] (ade6+ and ade7+).
  6. f. Not determinable; no associated terms or categories in AmiGO or KEGG.