Quantification of the expression of selected genes by RT-qPCR. Ordinate, relative copy number (n, 3 each; ± S.E.M.) determined in the short time waiting experiment. Open bars: unstimulated control, filled bars: primed cells, light sanded bars: E. coli induction of naïve cells; dark shaded bars: E. coli induction of primed cells. Fold changes and significance (* p < 0.1, ** p < 0.05, *** p < 0.01, paired t-test) of the priming-related differential expression are indicated. All individual data are listed in the Additional file 4, Table S4.