Table 3 qRT-PCR primer sequences
Gene | Accession no. | Forward primer (3’ - 5’) | Reverse primer (5’ - 3’) | |
|---|---|---|---|---|
mt D Loop B (gDNA) | NC001960 | CCCCTGAAAGCCGAATGTAA | CGACCTTGTTAGACTTCTTTGCTTG | |
MyoD2 (gDNA) | AJ557150 | CAGAGCCAGGATTACACTCGTTACA | GCATGTCGCTGGTGTTGAAG | |
PPARα | DQ294237 | TCCTGGTGGCCTACGGATC | CGTTGAATTTCATGGCGAACT | |
PPARβ | AJ416953 | GAGACGGTCAGGGAGCTCAC | CCAGCAACCCGTCCTTGTT | |
PPARγ | AJ416951 | CATTGTCAGCCTGTCCAGAC | TTGCAGCCCTCACAGACATG | |
EF1α | AF321836 | CACCACCGGCCATCTGATCTACAA | TCAGCAGCCTCCTTCTCGAACTTC | |
MYH6 | DW559270 | CAGGTCCTCTATGTGCTGGTGTG | TCCTCATTGTAGTTGCTGTCCTCAC | |
ANGL4 | GRASP209147493 | CCGTATGGGGGATGATGCTAA | GGTAGTATGCTGACGACTGACACCT | |
GTR1 | S30269700 | GCCATGGATGTCCTACGTGA | CTCCGCTACATACGGGAAGG | |
CPT1A | S31823509 | TCCCACATCATCCCCTTCAACT | TGTCCCTGAAGTGAGCCAGCT | |
ACADS | S31978689 | CTGGGGAAGAAGGAGGACAAG | TCTAGAGCAGCCTGAGCAATACC | |
NKX2.5 | DW550500 | CCCAGTACGTCCACACCCTT | GGAGGTCGGTAAGGCACAGT | |
Mef2C | GU252207 | CACCGTAACTCGCCTGGTCT | GCTTGCGGTTGCTGTTCATA | |
GATA4 | HM475152 | TCTCCATTCGACAGCTCCGT | CATCGCTCCACAGTTCACACA | |
Osx | FJ195614 | ATTACTGAGGAGGAGCCCATCATT | CCTCATCCACCTCACACACCTT |