A novel method of identifying W-specific contigs. (A) The steps in our classification procedure. (B) Alignment results were summarized by two statistics: coverage and read depth. If a contig consists of unique sequence (solid black) and masked repetitive regions (hatched), then coverage is the proportion of unique sequence covered by a read. Read depth is the number of reads divided by the total possible locations to which a read could map. (C) Predicted alignment results. Each W-specific contig should have very few male-derived reads uniquely aligning to it.