Figure 5
Distribution of probe sets hybridized predominantly with specific and non-specific transcripts as a function of the position of the first (left part) and last (right part) probe of each probe set. The graphs show that ‘short’ probe sets located nearer to the 3′-end of the transcript are more prone to bind non-specific transcripts at (L1 < 100 and Lmax < 500) than specific ones. The part in the middle shows the difference between the respective fractions of probe sets whereas the part above normalizes this difference with respect to the mean fraction of probe sets in both, S- and N-groups. Accordingly, the differential binding refers to about 50% of all probe sets. The distributions are calculated using the ratQC data set