Figure 2

Overlap of SYT-SSX2 peaks with epigenetic markers. Datasets that identified regions of enrichment for histone modifications and PolII binding [28] were compared with the locations of the SYT-SSX2 peaks using the Galaxy analysis suite to determine sites of overlap. The bar graph shows the percent of SYT-SSX2 peaks that overlap with 10 epigenetic markers. Ubiquitylated histone H2 lysine 120 (H2UB) is a marker for transcription elongation. Mono, di, and trimethylated histone H3 lysine 4 (H3K4me1, H3K4me2, H3K4me3), as well as acetylated histone H3 lysine 9 (H3K9Ac), acetylated histone H3 lysine 18 (H3K18Ac), acetylated histone H4 lysine 12 (H4K12Ac), and RNA Polymerase II (PolII) all mark gene promoters and enhancers. Trimethylated histone H3 lysine 36 (H3K36me3) is a marker for active transcription in gene bodies. Trimethylated histone H3 lysine 27 (H3K27me3) is the Polycomb-specific modification associated with chromatin silencing.