Figure 5

Models of SYT-SSX2 recruitment and activity. A)Model of recruitment and activity at an upregulated gene. SYT-SSX2 is recruited primarily to silent genes involved in the specification of other lineages by interactions with Polycomb Repressive Complexes (PRC1, dark blue oval; PRC2, light blue oval). In the presence of certain lineage-specific transcription factors (LSTF, red oval) and/or by interaction with lineage-specific enhancers (Enhancer, orange rounded rectangle), SYT-SSX2 association with the gene results in transcriptional activation. B) Model of recruitment and activity at a downregulated gene. Top) SYT-SSX2 may be recruited by interactions with Polycomb Repressive Complexes at a distance from the gene TSS. The gene itself is marked by activating histone modifications (Active, pink circle), and due to the presence of other regulators (?, black rounded rectangle), SYT-SSX2 carries out transcriptional silencing. Bottom) SYT-SSX2 may be directly recruited to the proximal regulatory region through association with activating histone modifications or other unidentified factors. These other factors also provide a signal to SYT-SSX2 such that it mediates repression.