Table 4 Predicted heme and cobalamin biosynthesis genes on the G. lovleyi strain SZ genome in comparison to the model organism for anaerobic cobalamin biosynthesis, Salmonella enterica subsp. enterica ser. Typhimurium
| Â | G. lovleyi SZ genome | Homolog in Salmonella enterica subsp. enterica ser. Typhimurium LT2 | |||
|---|---|---|---|---|---|
Chromosome | Plasmid pSZ77 | Gene symbol | Function | % Ident. (%Simil.) | |
Chr.#pSZ77 | |||||
Early heme biosynthesis | Glov_0501 | Â | hemD | Uroporphyrinogen-III synthase | no match |
| Â | Glov_0502 | Â | hemC | Porphobilinogen deaminase | 52(65) |
| Â | Glov_0503 | Â | hemA | Glutamyl-tRNA reductase | 40(62) |
|  | Glov_0688 |  | hemB | δ-Aminolevulinic acid dehydratase | 47(63) |
Late heme biosynthesis | Glov_0521 | Â | hemY | Protoporphyrinogen oxidase | no match |
| Â | Glov_1228 | Â | hemN | Coproporphyrinogen III oxidase | 27(45) |
| Â | Glov_2930 | Â | hemH | Ferrochelatase | 31(47) |
| Â | Glov_3245 | Â | hemL | Uroporphyrinogen decarboxylase | 52(67) |
Cobalamin biosynthesis | Glov_3417 | Glov_3646 | cbiE | Precorrin-6y C5,15-methyl-transferase | 32(54)35(57) |
| Â | Glov_3647 | cbiJ | Precorrin-6x reductase | 30(44) | |
| Â | Glov_3648 | cbiH | Precorrin-3B C17-methyltransferase | 46(63) | |
| Â | Glov_3649 | cbiG | Cobalamin biosynthesis protein | 31(49) | |
| Â | Glov_3650 | cbiF | Precorrin-4 C11-methyltransferase | 50(68) | |
| Â | Glov_3651 | cbiD | Cobalamin biosynthesis protein | 35(51) | |
| Â | Glov_3652 | cbiL | Precorrin-2 C20-methyltransferase | 33(52) | |
| Â | Glov_3653 | cbiK | Anaerobic cobalt chelatase | 32(45) | |
| Â | Glov_3654 | cbiC | Precorrin-8X methylmutase | 34(52) | |
| Â | Glov_3655 | cbiA | Cobyrinic acid a,c-diamide synthase | 39(55) | |
| Â | Glov_3656 | sirA/cysG | Siroheme synthase/ uroporphyrin-III C-methyltransferase | 41(57)/40(57) | |
Glov_2767 | Glov_3657 | cbiO | Cobalt ABC transporter, ATPase subunit | 38(54) 35(56) | |
Glov_2768 | Glov_3658 | cbiQ | Cobalt ABC transporter, inner membrane subunit | 26(44) 23(45) | |
| Â | Glov_3659 | cbiN | Cobalt transport protein | 42(57) | |
Glov_2769 | Glov_3660 | cbiM | Cobalt transport protein | 35(48) 54(68) | |
Glov_0692 | Glov_3718 | cobA | Corrinoid:ATP adenosyltransferase | 36(54) 46(67) | |
Glov_3081 | Â | cobU | Bifunctional adenosylcobinamide kinase /adenosylcobinamide-phosphate guanylyltransferase | 45(57) | |
Glov_3082* | Glov_3678 | cobT | Nicotinate-nucleotide-DMBA-phosphoribosyltransferase | 38(57) 39(57) | |
Glov_3083 | Â | cobS | Cobalamin synthase | 35(51) | |
|  | Glov_3084 |  | cobC | α-Ribazole-5'-phosphate phosphatase | 25(43) |
| Â | Glov_3417 | Â | cbiT | Precorrin decarbocylase | 33(54) |
Glov_3553 | Â | cbiB | Cobalamin biosynthesis protein | 46(62) | |
Glov_3554* | Glov_3679 | cobD/cbiP | L-threonine O-3-phosphate decarboxylase/ cobyric acid synthase | 34(51)/45(63) | |
45(62)/35(51) | |||||