Figure 3

Expression patterns of hyperoxia-responsive miRNA and miRNA targets. Total RNA was isolated and RNA quality confirmed. In total, 12 samples were used for miRNA profiling, 2 samples for each normoxia group at time points of P1, P14, and P29; and 3 samples for each hyperoxia-treated group at time points P14 and P29. MiRNA expression profiling was performed using the RT-PCR based Taqman Rodent MicroRNA array measuring 521 mature mouse miRNAs. Raw miRNA array data were analyzed using RQ manager software. (A) 6 expression patterns in 3 groups represented by color heat maps. (B) MiRNA expression and prediction of miRNA targets. Average miRNA expression is shown as copy number. Predicted targets indicate miRNA targets identified by overlapping computationally predicted mRNA targets with the mRNAs having opposite expression patterns with their corresponding individual miRNAs in our data. When two mature miRNAs originate from opposite arms of the same pre- miRNA, they are denoted with a -3p or -5p suffix. When relative expression levels are known, an asterisk following the name indicates a miRNA expressed at low levels relative to the miRNA in the opposite arm of a hairpin, for example, miR-322 and miR-322* would share a pre-miRNA hairpin, but more miR-322 would be found in the cell [24].