Expression of transcription factors. (A) Average normalized read counts for members of transcription factor families at four stages of A. queenslandica development. Genes were retrieved from previous studies [23, 56, 57]. (B) Gene families exhibiting coordinated gene expression. Short chain collagens, cadherin domain-containing, and scavenger receptor domain-containing gene families show specific upregulation at the adult stage. Heat maps show the relative expression of these genes across development (red, high; blue, low). Stage enrichment of genes was estimated using Fisher’s exact test (p-values shown). (C) Cluster of 5 scavenger receptor domain-containing genes that are expressed in the adult sponge. Reads mapping to this genomic locus in the different developmental stages are shown (reads normalized to 10 million, maximum peak height shown is 100 reads). Non-scavenger receptor genes in the cluster are shown in gray.