Figure 4

Msex\Orco expression in larval antennae is variably reduced following dsRNA treatment. Relative expression of Msex\Orco was measured by quantitative RT-PCR in male larval antennae 3 days after dsRNA delivery, and here is shown as a percentage of expression compared with paired control samples (dashed line indicates expression in control samples, normalized to 100%). Note that in half of trials, Msex\Orco expression was similar in treated and control animals (trials 6–10) while in the other half, Msex\Orco expression is markedly reduced following dsRNA treatment (trials 1–5). Cumulatively, dsRNA treatment results in significant reduction of Msex\Orco expression (p = .03, paired, one-tailed t-test). For quantitative RT-PCR, relative expression of Msex\Orco was measured and normalized to an endogenous control (rpS3) as described in the Experimental Procedures and shown in Additional file 3: Table S.sx 1