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Table 2 Essential genes in E. coli that are not as strongly selected in Typhimurium or Typhi*

From: High-throughput comparison of gene fitness among related bacteria

S. Typhimurium 14028 gene symbol

Gene name

Gene description

Best hit in S. Typhimurium LT2

Best hit in S. Typhi Ty2a

Best hit in ECOa

STM1 transposons

STM1 reads

STY1 transposons

STY1 reads

STY2 transposonsb

STY2 readsb

STY2 transposonsc

STY2 readsc

      

Percentile rank

STM14_0106

folA

Dihydrofolate reductase

STM0087

t0090

b0048

4

3

8

8

19

19

46

60

STM14_0217

folK

2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase

STM0183

t0191

b0142

12

22

18

16

25

56

16

21

STM14_2754

yejM

Putative hydrolase

STM2228

t0626

b2188

14

16

30

25

13

19

17

20

STM14_4041 d

degS

Serine endoprotease

STM3349

t3265

b3235

50

34

14

14

6

7

4

4

STM14_4193

trpS

Tryptophanyl-tRNA synthetase

STM3481

t4024

b3384

18

16

14

14

78

64

12

12

STM14_4208

yrfF

Intracellular growth attenuator protein

STM3495

t4011

b3398

1

4

19

19

70

42

12

27

STM14_4460

gpsA

NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

STM3700

t3819

b3608

10

10

14

17

45

46

8

11

STM14_4921

ftsN

E cell division protein

STM4093

t3525

b3933

42

38

14

15

20

27

15

15

STM14_4971.J

murB

UDP-N- acetylenolpyruvoylglucosamine reductase

STM4136

t3489

b3972

42

48

26

22

8

24

13

21

  1. * Details for all genes can be found in Additional file5: Table S2. Genes were ranked by the number of transposon insertions and number of independent reads per gene. Ranks above the 15th percentile are shown in bold.
  2. a Best hits are listed only if gene is syntenic with S. Typhimurium strain 14028 and has at least 95% sequence identity.
  3. b Growth on L-agar with “aro mix” (L-phe, L-trp, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid)[3].
  4. c Growth after six passages in LB broth[3].
  5. d Considered essential in STY2[3].