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Figure 2 | BMC Genomics

Figure 2

From: Insights into the evolution of mammalian telomerase: Platypus TERT shares similarities with genes of birds and other reptiles and localizes on sex chromosomes

Figure 2

Platypus alternatively spliced TERT variants. (a) The comparison of platypus, chicken, and human AS variants. The figure show all identified platypus variants, and all chicken and human variants that involved the second exon. Additionally, the chicken and human variants most similar to platypus C variant are shown. The structure of wild-type (WT) platypus, chicken, and human TERT is shown at the top of each set of AS variants. The TERT ORFs are shaded. The positions of the exons and functional domains (TEN, TRBD, RT, CT) of TERT are indicated. The model of TERT transcripts with single alternative splice events are drawn to their relative sizes. Deletions (triangles) and insertion (gray rectangle) resulting from splicing events are indicated. The red triangles represent AS variants conserved between platypus and chicken. Blue triangle identifies AS TERT variant which contains a deletion of a similar RT region in platypus, chicken, and human TERT. The black triangles indicate AS variants which are not conserved among platypus, chicken, and human. Asterisks identify the location of the stop codons. The descriptive and trivial names of the TERT AS sequences are shown in the right margin. The numbers in the parentheses represent the position of the deletion in the exon which is indicated in front of these parentheses. The figure is based on data from the following GenBank entries of platypus isoforms: [GenBank:JF441069] (A), [GenBank:JF441066] (A3), [GenBank:JF441065] (A2), [GenBank:JF441068] (pA4), [GenBank:JF441070] (B), [GenBank:JF441067] (C), [GenBank:JF441072] (D); chicken isoforms: [GenBank:JF896279] (A2), [GenBank:DQ148473] (A), [GenBank:DQ681311] (A3), [GenBank:DQ256211] (V3), [GenBank:DQ148464] (G), and human isoforms: [GenBank:AB086379] (Δ7-8), and human isoforms [GenBank:JF896280] (Δ2), [GenBank:JF896283] (Δ2-8), [GenBank:JF896284] (Δ2-13). The available sequence of human Δ2p(136-end) variant is too short to be accepted by GenBank and is provided in the supplement (Additional file 3, p.5). (b) The ClustalX alignment of amino acid sequences encoded by the second exon of platypus (Oan), domestic chicken (Gga), Japanese quail (Cja), human (Hsa), and mouse (Mmu) TERT. The part of TEN domain present in the second exon is shown at the top. The novel SA and SD sites are indicated by small boxes. For mouse AS TERT variants see [GenBank:CF531121.1, GenBank:BC127068.1]. The prefixes to SD or SA represent species: Oan (platypus), ch (chicken), q (quail), h (human), and m (mouse). The name of the relevant platypus or chicken alternative splicing variant(s) is indicated after the SD and SA used to create this isoform(s). The residues shown by (*) are conserved in all species.

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