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Figure 5 | BMC Genomics

Figure 5

From: Insights into the evolution of mammalian telomerase: Platypus TERT shares similarities with genes of birds and other reptiles and localizes on sex chromosomes

Figure 5

Chromosomal localization of the Oan TERT locus. (a) Fluorescence in situ hybridization (FISH) of TERT-containing BAC clone on male platypus metaphase spread. Green fluorescence signals indicate the position of the TERT locus on a pair of metacentric chromosomes (green arrows). Scale bar = 10 μm. (b) FISH of TERT (green) and the major histocompatibility complex (MHC) (red) was performed as described in (a). TERT signals were identified on the pseudoautosomal regions of Y2qX3p. (c) Schematic representation of platypus chromosomes X2, Y2, X3, Y3. Approximate positions of TERT and MHC signals are indicated. Locations of gene contigs Ultra 474 (U474, blue), Ultra 43 and X3 (U43-X3, purple) are indicated as determined in this (c) and previous works [54, 55]. (d) The analysis of shared synteny between TERT-containing chromosomal regions of platypus and human. The platypus genes located at platypus X2 and X3 chromosomes and their human orthologs (on human chromosomes) are indicated by distinct colored frames. The genes without the colored frame were not yet localized to platypus chromosomes. The numbers of the platypus gene contigs as present in current Ensembl database are indicated. “Ultra” in the designation of some contigs is shortened to “U”. The mutual orientation of platypus X3 and U43 contigs and their relative position on the X3 chromosome were not established so that the orientation shown in the panel C may not reflect their actual positioning and orientation. The genes located at different human chromosomes and their platypus orthologs (on platypus chromosomes) are indicated by distinct colored backgrounds. Light blue field identifies parts of human chromosomes that share synteny with chicken chromosome 2. Human genes located on 5p15 which have no platypus orthologs are indicated by gray lettering. The vertical arrows in the group of 6p24.3 genes and their platypus X2 orthologs represents a group of approximately 25 additional orthologous genes that extend shared synteny between 6p and X2.

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