Quantitative real time PCR copy number assays. Taqman assays were used to examine copy number variations at 10 locations within the CFTR gene in both the unamplified and amplified DNA samples. Average calculated copy number values are plotted with bars representing minimum and maximum values from replicate measurements (n = 4). Within each amplification / sample group, the order of the probe results is I- X (see Figure 4). UnAmp (no amplification); Amp-R (REPLI-g amplified); Amp-G (GenomiPhi amplified).