Figure 3
(A) Integrated genetic-RH-FISH map of the tilapia chromosome LG7. The RH map on the middle consists of three RH groups containing ordered markers whose coordinates are indicated in cR3500. Microsatellites (in blue) allowed the anchorage of the RH map to the genetic map [15] figured by a vertical bar on the left. Double-FISH of BAC clones highlighted by a red or green frame indicate the relative position of the RH groups on the chromosome symbolized on the right side. The chromosome is orientated with its centromere up. (B) Results of Double-FISH experiment of BAC clone WG0AAA35YD23HM1 revealed with FITC (green) and BAC clone WG0AAA16YE01HM1 revealed with Rhodamin (red) on a chromosome preparation. (C) Results of Double-FISH experiment of BAC clone WG0AAA35YD23HM1 revealed with FITC (green) and BAC clone WG0ACA29YJ13M1 revealed with Rhodamin (red) on a chromosome preparation.