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Table 1 KL1 genome annotation

From: Comparative analysis of two phenotypically-similar but genomically-distinct Burkholderia cenocepacia-specific bacteriophages

Gene

Start

End

Putative function

Strand

Predicted ribosome binding site and start codon

Length (amino acids)

Closest relative

Alignment region (amino acids)

Percent identity

Source

GenBank accession number

1

1

267

unknown

+

AGGGGCGAActtcgtATG

88

hypothetical protein ORF001

1-84/84

77

Pseudomonas phage 73

YP_001293408.1

2

264

560

holin

+

AAAGGGGCGGtaacGTG

98

hypothetical protein ORF002

3-88/88

42

Pseudomonas phage 73

YP_001293409.1

3

514

1080

lysin

+

AAAAGGGGttatcgaATG

188

hypothetical protein bglu_1g27070

2-181/188

47

Burkholderia glumae BGR1

YP_002912484.1

4

1091

1408

Rz

+

AAGTAAGGGGttcgaaATG

105

hypothetical protein ORF004

1-101/101

37

Pseudomonas phage 73

YP_001293411.1

5

1329

1592

Rz1

+

GAAAGGtgccgccgATG

87

conserved hypothetical protein

1-79/86

40

Burkholderia sp. Ch1-1

ZP_06842908.1

6

1647

2138

unknown

+

ACTAGGccgcgattATG

163

hypothetical protein ORF005

1-162/162

59

Pseudomonas phage 73

YP_001293412.1

7

2116

3756

terminase large subunit

+

AACAGGAAttgcttaATG

546

hypothetical protein ORF006

10-531/531

84

Pseudomonas phage 73

YP_001293413.1

8

3770

5266

portal protein

+

AAAGGAAAcgaaatcATG

498

hypothetical protein ORF007

3-494/501

85

Pseudomonas phage 73

YP_001293414.1

9

5269

6384

head morphogenesis protein

+

GGGGCGTAatcATG

371

hypothetical protein ORF008

1-364/364

73

Pseudomonas phage 73

YP_001293415.1

10

6403

7110

unknown

+

AAGGAGtccttgaaATG

235

hypothetical protein ORF009

1-235/239

82

Pseudomonas phage 73

YP_001293416.1

11

7123

8097

major capsid protein

+

AAGGAcactttatcATG

324

hypothetical protein ORF010

1-325/325

90

Pseudomonas phage 73

YP_001293417.1

12

8171

8587

unknown

+

AAGGAGtttcgaacATG

138

hypothetical protein ORF011

1-134/134

69

Pseudomonas phage 73

YP_001293418.1

13

8656

9033

unknown

+

AAAGGAGcgtcgaacATG

125

hypothetical protein ORF012

1-123/123

70

Pseudomonas phage 73

YP_001293419.1

14

9047

9565

unknown

+

AAGGGGcgcggcatcATG

172

hypothetical protein ORF013

1-172/172

83

Pseudomonas phage 73

YP_001293420.1

15

9570

9944

head-tail joining protein

+

GATAAGGGtctaacgctATG

124

hypothetical protein ORF014

1-124/126

59

Pseudomonas phage 73

YP_001293421.1

16

9941

10399

minor tail protein

+

ATACGGTAttgttcgcacaATG

152

hypothetical protein ORF015

5-151/151

68

Pseudomonas phage 73

YP_001293422.1

17

10412

11965

unknown

+

AAGGAGttacgaaaATG

517

hypothetical protein ORF016

3-511/511

78

Pseudomonas phage 73

YP_001293423.1

18

12030

12458

tail protein

+

GGAGTAAAccaaATG

142

hypothetical protein ORF017

1-142/142

79

Pseudomonas phage 73

YP_001293424.1

19

12030

12823

tail protein

+

GGAGTAAAccaaATG

264

hypothetical protein ORF017

1-142/142

79

Pseudomonas phage 73

YP_001293424.1

       

hypothetical protein ORF018

1-118/118

78

Pseudomonas phage 73

YP_001293425.1

20

12792

13226

tail protein

+

AAAAGGCGGcgcaacagaATG

144

hypothetical protein ORF019

1-144/144

80

Pseudomonas phage 73

YP_001293426.1

21

13232

17050

tail tape measure

+

AAGGAttagcagaaATG

1272

hypothetical protein ORF020

1-78, 131-1202/1204

61, 57

Pseudomonas phage 73

YP_001293427.1

22

17069

18067

unknown

+

AGGAAtacgaattATG

332

hypothetical protein XALc_0225

1-295/307

30

Xanthomonas albilineans GPE PC73

YP_003374757.1

23

18070

19179

unknown

+

GAGGAAAActaatcATG

369

hypothetical protein ORF033

1-332/333

25

Pseudomonas phage M6

YP_001294541.1

24

19179

20870

tail assembly protein

+

AAGAAGAtcgcataATG

563

hypothetical protein ORF023

63-565/568

36

Pseudomonas phage 73

YP_001293430.1

25

20867

21688

tail assembly protein

+

AAGGAcgattccagaATG

273

hypothetical protein ORF024

1-273/274

49

Pseudomonas phage 73

YP_001293431.1

26

21689

24100

tail assembly protein

+

AAGATGGGGtcggttaaATG

803

hypothetical protein ORF025

1-755/813

49

Pseudomonas phage 73

YP_001293432.1

27

24097

26166

DNA polymerase

-

AAGGAAtttgcccgATG

689

hypothetical protein ORF026

1-682/683

83

Pseudomonas phage 73

YP_001293433.1

28

26179

27339

DNA polymerase III β subunit

-

AAGGGGttaaaaATG

386

hypothetical protein ORF027

2-380/380

74

Pseudomonas phage 73

YP_001293434.1

29

27323

27691

unknown

-

GAATGGtgaaattATG

122

hypothetical protein Dole_2913

5-84/87

33

Desulfococcus oleovorans Hxd3

YP_001530793.1

30

27696

29351

superfamily II helicase/restriction enzyme

-

AAGGGttacgaATG

551

hypothetical protein ORF029

1-551/551

90

Pseudomonas phage 73

YP_001293436.1

31

29344

30342

exonuclease

-

GGAAGGcgaagaacgATG

332

hypothetical protein ORF030

1-365/365

65

Pseudomonas phage 73

YP_001293437.1

32

30852

31637

unknown

-

GAAAGGtgaaacgaacATG

261

hypothetical protein Isop_2441

1-118/151

37

Isosphaera pallida ATCC 43644

YP_004179564.1

33

31696

32412

recombinase

-

AGGTGAAcgtATG

238

hypothetical protein ORF032

1-238/238

91

Pseudomonas phage 73

YP_001293439.1

34

32471

32980

unknown

-

AAGGAAccccaaaATG

169

hypothetical protein ORF033

7-146/146

49

Pseudomonas phage 73

YP_001293440.1

35

33059

33598

pyrophosphohydrolase

-

AGGGGcatcgtATG

179

hypothetical protein ORF034

8-185/185

69

Pseudomonas phage 73

YP_001293441.1

36

33746

33934

transcriptional regulator

+

GGGGcaagcATG

62

hypothetical protein ORF035

1-61/62

51

Pseudomonas phage 73

YP_001293442.1

37

33924

36233

primase

+

GAAGGcttgcgcaaatATG

769

hypothetical protein ORF036

1-773/773

85

Pseudomonas phage 73

YP_001293443.1

38

36366

36668

unknown

+

GAAGGAgttacgaacATG

100

hypothetical protein

132-217/217

44

Deftia phage Ï•W-14

YP_003359005.1

39

36735

37091

unknown

+

GAAGGAGtacacgccATG

118

unnamed protein product

262-336/404

32

Azospirillum lipoferum 4B

YP_004974060.1

40

37097

37360

unknown

+

AGAAGAAGGAGtaagcgccATG

87

PREDICTED: photosystem II reaction center PSB28 protein, chloroplastic

22-86/179

32

Vitis vinifera

XP_002271666.1

41

37728

38024

unknown

+

AAAGGAGcgccagccATG

98

hypothetical protein ORF039

1-97/98

70

Pseudomonas phage 73

YP_001293446.1

42

38060

38296

unknown

+

AAGGAAccccgatcATG

78

hypothetical protein ORF040

1-80/80

50

Pseudomonas phage 73

YP_001293447.1

43

38302

38703

unknown

+

AAAGGGGtaattactATG

133

hypothetical protein ORF042

1-120/124

40

Pseudomonas phage 73

YP_001293449.1

44

38707

39195

Vsr endonuclease

+

GACGAAGttgcattaagccATG

162

hypothetical protein ORF043

1-176/179

61

Pseudomonas phage 73

YP_001293450.1

45

39201

39458

unknown

+

GGAAGGAGtaacccaaATG

85

hypothetical protein Astex_0306

3-81/183

44

Asticcacaulis excentricus CB 48

YP_004086155.1

46

39455

39655

unknown

+

GGCGAAGtcgtcgaATG

66

monooxygenase, FAD-binding

385-445/546

38

Streptomyces griseoflavus Tu4000

ZP_07309792.1

47

39652

39840

unknown

+

AAGGAGtacgcaccATG

62

hypothetical protein METUNv1_00516

11-65/68

39

Methyloversatilis universalis FAM5

ZP_08503515.1

48

39882

40154

unknown

+

AAAAGGAGtaacgaacATG

90

hypothetical protein Cflav_PD2164

58-133/172

30

bacterium Ellin514

ZP_03630603.1

49

40138

40374

unknown

+

GAACCGGAttacgattATG

78

hypothetical protein ORF047

2-77/77

67

Pseudomonas phage 73

YP_001293454.1

50

40374

40550

unknown

+

GGGTTAcgaataATG

58

hypothetical protein Glaag_3667

90-140/227

29

Glaciecola sp. 4 H-3-7 + YE-5

YP_004435864.1

51

40562

40933

unknown

+

GAAAGGtgaaatcATG

123

hypothetical protein BURMUCGD2M_4586

8-67/70

34

Burkholderia multivorans CGD2M

ZP_03569237.1

52

40930

41415

dCMP deaminase

+

GGAACGtccggcATG

161

hypothetical protein ORF049

2-153/155

75

Pseudomonas phage 73

YP_001293456.1

53

41412

41786

unknown

+

AAAGGctgaatcATG

124

hypothetical protein ORF050

4-125/127

43

Pseudomonas phage 73

YP_001293457.1

54

41826

42032

unknown

+

GGGGAtgcccacattATG

68

hypothetical protein ORF051

37-94/94

45

Pseudomonas phage 73

YP_001293458.1

55

42120

42674

unknown

+

AAGGAGttttacaaATG

184

hypothetical protein ORF052

9-190/190

66

Pseudomonas phage 73

YP_001293459.1