Table 1 Gene functions of the Vibrio phage ϕpp2

Feature ID

Start

Stop

nt (bp)

aa

Function

Match to

Color

fig 75320.3.peg.1

41

973

933

311

RNaseH ribonuclease

KVP40 & T4

G*

fig 75320.3.peg.3

1318

1611

294

98

late promoter transcription accessory protein

KVP40

Y

fig 75320.3.peg.6

3216

3761

546

182

Frd dihydrofolate reductase

KVP40 & T4

G

fig 75320.3.peg.7

3758

4477

720

240

ATP-dependent Clp protease proteolytic subunit (EC3.4.21.92)

KVP40

Y

fig 75320.3.peg.8

4544

5644

1101

367

Phage recombination protein

KVP40 & T4

G

fig 75320.3.peg.10

6060

7343

1284

428

DNA primase-helicase subunit

KVP40 & T4

G

fig 75320.3.peg.12

7583

9418

1836

612

Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)

KVP40 & T4

G

fig 75320.3.peg.15

10506

10982

477

159

Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4)

KVP40 & T4

G

fig 75320.3.peg.16

10982

11506

525

175

putative serine/threonine protein phosphatase

KVP40

Y

fig 75320.3.peg.18

12420

13244

825

275

98.1% KVP40 DNA helicase, phage-associated

KVP40

Y

fig 75320.3.peg.19

13244

13720

477

159

gp61.1 conserved hypothetical

KVP40 & T4

G

fig 75320.3.peg.20

13801

14859

1059

353

DNA primase (EC 2.7.7.-)/DNA helicase (EC 3.6.1.-). Phage-associated

KVP40 & T4

G

fig 75320.3.peg.21

14859

15356

498

166

Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23)

KVP40

Y

fig 75320.3.peg.23

15589

16281

693

231

exonuclease A

KVP40 & T4

G

fig 75320.3.peg.31

18182

19081

900

300

Thymidylate synthase (EC 2.1.1.45)

KVP40

G

fig 75320.3.peg.42

23359

24024

666

222

NAD-dependent protein deacetylase of SIR2 family

KVP40

Y

fig 75320.3.peg.43

24180

25973

1794

598

DNA gyrase subunit B (EC 5.99.13)

KVP40 & T4

G

fig 75320.3.peg.49

27350

28078

729

243

Ser/Tar protein phosphatase family protein

KVP40

Y

fig 75320.3.peg.55

30282

31625

1344

448

DNA ligase

KVP40 & T4

G

fig 75320.3.peg.60

36003

36242

240

80

glutaredoxin

KVP40

Y

fig 75320.3.peg.61

36301

37197

897

299

Phage capsid vertex protein (T4-like gp24)

KVP40 & T4

G

fig 75320.3.peg.62

37206

37718

513

171

T4-like phage RNA polymerase sigma factor for late transcription # T4-like phage gp55#T4GC0140

KVP40 & T4

G

fig 75320.3.peg.68

41040

41504

465

155

gp30.3

KVP40 & T4

G

fig 75320.3.peg.69

41509

41994

486

162

Putative 5'(3')-deoxyribonucleotidase (EC 3.1.3.-)

KVP40

Y

fig 75320.3.peg.70

41991

43031

1041

347

Phage recombination-related endonuclease Gp47

KVP40 & T4

G

fig 75320.3.peg.72

43248

45485

2238

746

recombination endonuclease subunit

KVP40 & T4

G

fig 75320.3.peg.75

46247

46912

666

222

27.11% T4 Sliding clamp DNA polymerase accessory protein, phage associated # Gp45

T4

DG

fig 75320.3.peg.76

46983

47939

957

319

Replication factor C small subunit/Phage DNA polymerase clamp loader subunit # T4-like phage gp44 # T4 GC0157

KVP40 & T4

G

fig 75320.3.peg.77

47950

48438

489

163

31.11% T4 Phage DNA polymerase clamp loader (fig|10665.1.peg.49)

T4

DG

fig 75320.3.peg.78

48473

48853

381

127

RegA translaticnal repressor of early genes

KVP40 & T4

G

fig 75320.3.peg.79

49482

48856

627

209

MobE homing endonuclease

T4

R

fig 75320.3.peg.81

50130

52682

2553

851

DNA polymerase

KVP40 & T4

G

fig 75320.3.peg.83

53047

54192

1146

382

Rn1A

KVP40 & T4

G

fig 75320.3.peg.88

55585

56742

1158

386

3'-phosphatase, 5'-polynucleotide kinase, phage-associated #T4-like phage Pset #T4 GC1648

KVP40 & T4

G

fig 75320.3.peg.116

69766

70218

453

151

CMP/dCMP deaminase, zinc-binding

KVP40 & T4

G

fig 75320.3.peg.117

70273

71199

927

309

NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.-)

KVP40

Y

fig 75320.3.peg.118

71268

71942

675

225

GTP cyclohydrolase I (EC 3.5.4.16) type 1

KVP40

Y

fig 75320.3.peg.119

72615

71914

702

234

Phage-associated homing endonuclease

T4

R

fig 75320.3.peg.121

74083

74991

909

303

NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.-)

KVP40

Y

fig 75320.3.peg.122

75047

75763

717

239

Queuosine Biosynthesis QueC ATPase

KVP40

Y

fig 75320.3.peg.124

76380

76655

276

92

32.1% T4 Phage tail fibers

T4

DG

fig 75320.3.peg.127

77160

77498

339

113

Phage capsid and scaffold

KVP40

Y

fig 75320.3.peg.129

78455

77946

510

170

gp49 recombination endonuclease VII

KVP40 & T4

G

fig 75320.3.peg.131

78799

79806

1008

336

RNA ligase, phage-associated

KVP40 & T4

G

fig 75320.3.peg.141

83194

85263

2070

690

Phage rIIA lysis inhibitor

KVP40 & T4

G

fig 75320.3.peg.142

85256

86293

1038

346

rIIB protector from prophage-induced early lysis

KVP40 & T4

G

fig 75320.3.peg.148

89179

90216

1041

347

NrdC 1 1 conserved hypothetical protein.

KVP40 & T4

G

fig 75320.3.peg.151

90946

92211

1266

422

Dda DNA helicase

KVP40 & T4

G

fig 75320.3.peg.157

94752

95777

1026

342

Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1)/ADP-ribose pyrophosphatase

KVP40

Y

fig 75320.3.peg.188

105782

106366

585

195

Thymidine kinase (EC 2.7.1.21)

KVP40 & T4

G

fig 75320.3.peg.201

111295

111711

417

139

endonuclease

KVP40 & T4

G

fig 75320.3.peg.208

113557

114537

981

327

Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1)/Ribosyln:cotinamide kinase (EC 2.7.1.22)

KVP40

Y

fig 75320.3.peg.212

115492

116157

666

222

Ribosyl nicotinamide transporter, PnuC-like

KVP40

Y

fig 75320.3.peg.249

130608

131138

531

177

Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, comp

KVP40

Y

fig 75320.3.peg.255

134377

135366

990

330

moa A/nifB/pqqE family protein

KVP40

Y

fig 75320.3.peg.256

136483

135359

1125

375

moa A/nifB/pqqE family protein

KVP40

Y

fig 75320.3.peg.260

138690

140183

1494

498

Nicotinamide phosphoritosyltransferase (EC 2.4.2.12)

KVP40

Y

fig 75320.3.peg.268

144971

143919

1053

351

moaA/nifB/pqqE family protein

KVP40

Y

fig 75320.3.peg.273

147028

149253

2226

742

Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)

KVP40 & T4

G

fig 75320.3.peg.274

149293

149964

672

224

Phage-associated homing endonuclease

T4

R

fig 75320.3.peg.275

149957

151081

1125

375

Ribonucleotide reductase of class Ia (aerobic), beta subunit (EC 1.17.4.1)

KVP40 & T4

G

fig 75320.3.peg.276

151083

151382

300

100

NrdC thioredoxin

KVP40 & T4

G

fig 75320.3.peg.279

152313

153317

1005

335

Thioredoxin, phage-associated

KVP40 & T4

G

fig 75320.3.peg.280

153363

154649

1287

429

gp52 topoisomerase II medium subunit

KVP40 & T4

G

fig 75320.3.peg.282

154900

155781

882

294

Queuosine Biosynthesis QueE Radical SAM

KVP40

Y

fig 75320.3.peg.293

160936

161235

300

100

anti-sigma70 protein

KVP40

Y

fig 75320.3.peg.296

165535

162206

3330

1110

Phage tail fibers (Match to KVP40 peg.297)

KVP40

Y

fig 75320.3.peg.297

168882

165607

3276

1092

tail fiber fragment

KVP40

Y

fig 75320.3.peg.322

184667

184212

456

152

gp57B conserved hypothetical protein

KVP40 & T4

G

fig 75320.3.peg.324

185581

184943

639

213

dNMP kinase

KVP40 & T4

G

fig 75320.3.peg.325

186347

185814

534

178

gp3 tail completion and health stabilizer protein

KVP40 & T4

G

fig 75320.3.peg.328

189332

188484

849

283

Phage baseplate hub

KVP40 & T4

G

fig 75320.3.peg.329

190090

189344

747

249

Phage baseplate-tail tube initiator

KVP40

Y

fig 75320.3.peg.330

190507

190094

414

138

55.97% T4 Phage DNA end protector during packaging

T4

DG

fig 75320.3.peg.331

191144

190689

456

152

Phage head completion protein

KVP40 & T4

G

fig 75320.3.peg.332

191211

192350

1140

380

Phage baseplate tail tube cap

KVP40 & T4

G

fig 75320.3.peg.333

192350

192928

579

193

Phage baseplate wedge

KVP40 & T4

G

fig 75320.3.peg.335

194207

195466

1260

420

Phage baseplate hub

KVP40 & T4

G

fig 75320.3.peg.337

195956

196252

297

99

PAAR

KVP40 & T4

G

fig 75320.3.peg.340

197717

198136

420

140

Phage baseplate wedge

KVP40 & T4

G

fig 75320.3.peg.341

198223

200181

1959

653

Phage baseplate wedge

KVP40 & T4

G

fig 75320.3.peg.342

200181

203678

3498

1166

Phage baseplate wedge initiator

KVP40 & T4

G

fig 75320.3.peg.343

203680

204702

1023

341

Phage baseplate wedge

KVP40 & T4

G

fig 75320.3.peg.344

204758

205714

957

319

gp9

KVP40 & T4

G

fig 75320.3.peg.345

205724

207970

2247

749

Phage baseplate wedge

KVP40 & T4

G

fig 75320.3.peg.348

210191

211612

1422

474

prophage LambdaSa04, minor structural protein, putative

KVP40 & T4

G

fig 75320.3.peg.349

211911

213590

1680

560

Phage neck whiskers

KVP40 & T4

G

fig 75320.3.peg.350

213601

214524

924

308

Phage neck protein

KVP40 & T4

G

fig 75320.3.peg.351

214528

215364

837

279

Phage neck protein

KVP40 & T4

G

fig 75320.3.peg.352

215593

216726

1134

378

tail health stabilizer and completion protein

KVP40 & T4

G

fig 75320.3.peg.354

217441

217989

549

183

Phage terminase, small subunit

KVP40 & T4

G

fig 75320.3.peg.355

217949

219751

1803

601

Phage terminase, large subunit

KVP40 & T4

G

fig 75320.3.peg.356

219798

221813

2016

672

Phage tail health monomer

KVP40 & T4

G

fig 75320.3.peg.357

221864

222364

501

167

Phage tail fibers

KVP40 & T4

G

fig 75320.3.peg.358

222404

223951

1548

516

portal vertex protein of head

KVP40 & T4

G

fig 75320.3.peg.360

224131

224622

492

164

Phage capsid and scaffold

KVP40 & T4

G

fig 75320.3.peg.361

224625

225266

642

214

Phage prohead core scaffold protein and protease

KVP40 & T4

G

fig 75320.3.peg.362

225299

226141

843

281

Phage scaffold prohead core protein

KVP40 & T4

G

fig 75320.3.peg.363

226212

227756

1545

515

Phage major capsid protein

KVP40 & T4

G

fig 75320.3.peg.364

228910

227813

1098

366

PSI-BLAST tRNA nucleotidyltransferase (Acinetobacter baumannii & Pseudomonas fluorescens)

KVP40 & T4

G

fig 75320.3.peg.367

230824

231315

492

164

Inh

KVP40 & T4

G

fig 75320.3.peg.377

236908

238431

1524

508

DNA helicase, phage-associated

KVP40 & T4

G

fig 75320.3.peg.381

239752

239339

414

138

UvsY recombination, repair and ssDNA binding protein

KVP40

Y

fig 75320.3.peg.383

246382

242612

3771

1257

gp34 long tail fiber, proximal subunit

KVP40

Y