Hybridization of subtelomeric genes with T. cruzi chromosomal bands. A. CL Brener molecular karyotype obtained after separation of chromosomal bands by PFGE (1.1% agarose gel stained with ethidium bromide). The size of each chromosomal band (Mb) is indicated on the right. In accordance with the nomenclature proposed by Cano and coworkers , the chromosomal bands are designated by Roman numerals, starting with the smallest band. B. Hybridization of the chromosomal bands with subtelomeric genes: TS, trans-sialidase; RHS, retrotransposon hot spot; DGF-1, dispersed gene family-1; C. Hybridization of the chromosomal bands with a single copy gene control (Histone H2B).