Figure 2

Arrayed quantification of miRNAs using a 96 well format. A miR-Q array layout is exemplified in Figure 2A using a 96-well format. This layout provides triplicate quantification of 26 miRNAs, 5 reference snRNAs and negative controls (NC). Figure 2B shows the C_q variations of triplicate measurements using 1 μg total RNA from differentiated THP-1. Rhombi indicate the individual measurements of each assay, while their mean is given as black lines. Black rhombi represent assays possessing specific products and blue rhombi show unspecific detection. The SYBR Green background is given as red dotted line.