Figure 5

Analytical specificity of miR-Q arrays. Figure 5A shows the alignment of miR-29 as well as miR-30 family members. Dashed borders indicate binding sites of 'miRNA'-rev primers. The percentage of cross reaction is given between assay-specific and unspecific miRNA targets. Assay-specific targets represent the full value (Figure 5B). The design of the miR-Q approach allows simultaneous quantification of a canonical miRNA as well as its isomiRs as exemplified by miR-24 (Figure 5C). The columns in Figure 5C show triplicate determination of the linear range using synthetic miR-24 as well as synthetic isomiRs. Pearson correlation analysis revealed high linearity between all data sets possessing r < 0.9969 and two-tailed P values < 0.0001 (Figure 5D).