Figure 2

Images of the 2-D gels of protein extracts from yeast cells. The 2-D gels were loaded with 200 μg of protein extract from the control transformant (A) or the Yap1p-overexpressing transformant (B). Proteins were visualized by fluorescence staining (Sypro Ruby). Typical data from triplicate 2-D gel images are shown in C (proteins involved in carbon metabolism) and D (proteins involved in pathways other than carbon metabolism). Histograms show the protein abundance, with the spot volume of the 2-D gels indicated in white (control) and black (Yap1p overexpression).