Transcriptional response mimicking either a high or a low glucose response. (A) Unsupervised hierarchical cluster diagram of all deletion mutants with gene expression changes differing from WT, i.e. twelve or more significant transcriptional changes, and all transcripts changing significantly in at least one of these mutants (p < 0.01, FC > 1.7). The dendrograms indicate relationships between transcripts (top) and mutants (right). The latter is colour-coded according to whether the mutants are part of the “high glucose” (red) or “low glucose” group (green). FC is indicated by the colour scale, with yellow for upregulation, blue for downregulation, and black for no change, versus the average WT. (B) Line graph of a time-course experiment in which glucose-depleted WT cells were inoculated into fresh media (SC, supplemented with 2% glucose) and their subsequent transcriptional output was monitored over a period of five hours. All transcripts differentially expressed between the “high glucose” and “low glucose” groups were split according to whether they were up- (left panel, yellow) or downregulated (right panel, blue) in the “low glucose” group. The average expression of the differentially expressed transcripts is indicated in black; all other transcripts are shown in grey.