Functional distribution of elm leaf EST matches from the five different single libraries based on their predicted Gene Ontology (GO) term. All GOs shown had at least 200 ESTs and an E value threshold of ≤1e-40 after annotation by the UniProt database. The GO terms from each library are divided into two groups: (A) important plant physiological processes and (B) defense-related processes including response to jasmonic acid (JA), ethylene (ET) and salicylate (SA). The libraries are: EF (egg laying & feeding), F (feeding), MeJA (methyl jasmonate), E (artificial scratching & egg transfer) and C (untreated control). The y-axis indicates relative EST matches by parts per thousand (ppt) relative to the library size. For statistical analysis EST abundances by library were compared pairwise by GO category. Asterisks denote treatments in which ESTs were differentially expressed relative to the control treatment; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; Fisher`s exact test.