An elm EST database for identifying leaf beetle egg-induced defense genes

Table 1 Sequencing output of elm libraries

cDNA Libraries^a	ESTs	Unitrans^b ≥ 2 ESTs	Library specific Unitrans^c	Singletons (%)^d
Untreated control (C)	2132	836	31	174 (17)
Egg & feeding (EF)	1921	826	50	211 (20)
Feeding (F)	4725	1453	65	326 (18)
Methyl jasmonate (MeJA)	7080	2272	153	679 (23)
Transferred eggs (E)	2133	811	40	188 (19)
Mix^e EF + F	169672	15083	2844	11560 (43)
C + MeJA + E	71239	9141	860	8043 (47)
C + EF + F + MeJA + E	98210	12402	2249	9755 (44)
Tag unidentifiable	4084	597	200	397 (40)
Total	361196	21490	–	31333 (59)

^a Libraries of differently treated elms: C = Control (untreated Ulmus minor leaves); EF = Egg laying & feeding (=leaves with elm leaf beetle eggs and feeding damage by female beetles [natural situation]; F = Feeding (=leaves with feeding damage by male beetles); Methyl jasmonate (= leaves treated with 50 μmol MeJA), E = Transferred eggs (= leaves that had been scratched and had eggs artificially placed on them ); ^b Number of Unitrans (unique transcripts) ≥2 ESTs that contain at least one EST of one of the libraries; ^c Number of Unitrans ≥2 ESTs that contain ESTs of only the particular library; ^d Percent singletons in relation to all unique transcripts (Unitrans ≥2 ESTs + singletons); ^e New sequencing runs of combined libraries.