An elm EST database for identifying leaf beetle egg-induced defense genes

BMC Genomics

Table 3 Identification of putative regulatory proteins with high occurrence in egg laying- and feeding-induced Ulmus minor leaves

Pfam accession	Gene description	# of ESTs	GO Biological process	Treatment (pptt)
				EF	EF+	E	F	MeJA	C	R
PF00305	Lipoxygenase	1602	lipid biosynthetic process	110	43	33	38	162	38	30.9
No family	Sieve element-occluding protein	1545	-	245	40	66	19	86	33	27
PF00199	Catalase	1159	response to stress	73	24	28	25	54	19	6.7
PF00160	Peptidyl-prolyl cis-trans isomerase	773	protein folding	52	15	9	19	34	33	5.3
PF00227	Proteasome subunit	341	response to stress	21	8	5	4	24	9	3.9
PF05564	Auxin-repressed protein	207	signal transduction	26	3	-	2	-	-	4.3
PF01717	Methionine synthase	200	methionine biosynthetic process	42	4	-	2	16	9	8.7
PF01641	Methionine sulfoxide reductase	58	catalytic activity	34	1	-	-	-	5	8.3
No family	Protein transport protein SFT2	16	vesicle-mediated transport	78	-	-	-	-	-	29.9

Treatments: C (untreated control), E (artificial scratching & eggs transferred), EF (egg deposition & feeding), F (feeding), MeJA (methyl jasmonate), mixed library EF + F. Relative Unitrans abundance calculated on counts by parts per ten thousand (pptt) based on the annotation to Plant UniProt (BLASTx, E-value ≤1e-20). Transcripts correlated on their predicted function to the Pfam = protein family database. R–values >3 were considered as significantly differentially expressed for the respective treatment against C (true positive rate of ~99%) by Test Statistics R [42].

ISSN: 1471-2164