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Table 4 Functional complementation of grapevine carotenoid biosynthetic genes in Escherichia coli

From: The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L.

Strain

Lycopene

δ-carotene

β-carotene

β-cryptoxanthin

zeaxanthin

Unknown

Total pigments

pAC-LYC + pGEM (control)

100.00

ND1

ND

ND

ND

ND

100.00

pAC-LYC + VvLBCY2

22.4

ND

72.1

ND

ND

5.5

100.00

pAC-LYC + VvLECY1

57.90

42.10

ND

ND

ND

ND

100.00

pAC-BETA + pGEM (control)

ND

ND

100.00

ND

ND

ND

100.00

pAC-BETA + VvBCH1

ND

ND

5.5

26.9

67.6

ND

100.00

pAC-85b + pGEM (control)

ND

ND

ND

ND

ND

ND

ND

pAC-85b + VvPSY1

ND

ND

100.00

ND

ND

ND

100.00

  1. 1 “ND”, not detected.
  2. Carotenoid accumulating E. coli cells were transformed with the respective cloned cDNA copies of the grapevine genes. The individual carotenoids analysed are expressed as percentages of the total pigments formed (standard error ≤ 5% (n ≥ 3).