Skip to main content

Table 4 Functional complementation of grapevine carotenoid biosynthetic genes in Escherichia coli

From: The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L.

Strain Lycopene δ-carotene β-carotene β-cryptoxanthin zeaxanthin Unknown Total pigments
pAC-LYC + pGEM (control) 100.00 ND1 ND ND ND ND 100.00
pAC-LYC + VvLBCY2 22.4 ND 72.1 ND ND 5.5 100.00
pAC-LYC + VvLECY1 57.90 42.10 ND ND ND ND 100.00
pAC-BETA + pGEM (control) ND ND 100.00 ND ND ND 100.00
pAC-BETA + VvBCH1 ND ND 5.5 26.9 67.6 ND 100.00
pAC-85b + pGEM (control) ND ND ND ND ND ND ND
pAC-85b + VvPSY1 ND ND 100.00 ND ND ND 100.00
  1. 1 “ND”, not detected.
  2. Carotenoid accumulating E. coli cells were transformed with the respective cloned cDNA copies of the grapevine genes. The individual carotenoids analysed are expressed as percentages of the total pigments formed (standard error ≤ 5% (n ≥ 3).