| | | |
Sequence of both sides
|
Sequence by a single termini
|
|---|
|
Strain
|
RFLP bands
|
PCR confirmed
|
#IS sites
|
False Positives
|
False Negatives
|
True Positives
|
#IS sites
|
False Positives
|
False Negatives
|
True Positives
|
|---|
|
Col-H37Rv
|
13
|
15
|
15
|
0
|
0
|
15
|
17
|
2
|
0
|
15
|
|
Col-UT98
|
11
|
13
|
10
|
0
|
3
|
10
|
13
|
0
|
0
|
13
|
|
Col-UT98C
|
11
|
13
|
10
|
0
|
3
|
10
|
13
|
0
|
0
|
13
|
|
Col-UT261
|
10
|
14
|
13
|
0
|
1
|
13
|
14
|
0
|
0
|
14
|
|
Arg-410
|
9
|
12
|
9
|
0
|
3
|
9
|
12
|
0
|
0
|
12
|
|
Arg-6548
|
8
|
11
|
8
|
0
|
3
|
8
|
11
|
0
|
0
|
11
|
|
Spa-C1
|
10
|
15
|
11
|
0
|
4
|
11
|
15
|
0
|
0
|
15
|
|
Total
|
72
|
93
| |
0
|
17
|
76
| |
2
|
0
|
93
|
|
Sensitivity
|
77%
| | | | |
82%
| | | |
100%
|
|
PPV*
| | | | |
100%
| | | | |
98%
|
- *PPV: Positive Predicted value.
- For each reference strain the number of RFLP observed bands is reported as well as the number of IS sites identified using IS-seq by one or both termini of the insertion and the number of confirmed sites by PCR. Sensitivity and Positive Predicted value is calculated as indicated in Methods.
