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Table 1 Accuracy of the IS-seq method with reference strains

From: IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes

   

Sequence of both sides

Sequence by a single termini

Strain

RFLP bands

PCR confirmed

#IS sites

False Positives

False Negatives

True Positives

#IS sites

False Positives

False Negatives

True Positives

Col-H37Rv

13

15

15

0

0

15

17

2

0

15

Col-UT98

11

13

10

0

3

10

13

0

0

13

Col-UT98C

11

13

10

0

3

10

13

0

0

13

Col-UT261

10

14

13

0

1

13

14

0

0

14

Arg-410

9

12

9

0

3

9

12

0

0

12

Arg-6548

8

11

8

0

3

8

11

0

0

11

Spa-C1

10

15

11

0

4

11

15

0

0

15

Total

72

93

 

0

17

76

 

2

0

93

Sensitivity

77%

    

82%

   

100%

PPV*

    

100%

    

98%

  1. *PPV: Positive Predicted value.
  2. For each reference strain the number of RFLP observed bands is reported as well as the number of IS sites identified using IS-seq by one or both termini of the insertion and the number of confirmed sites by PCR. Sensitivity and Positive Predicted value is calculated as indicated in Methods.