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Table 1 Accuracy of the IS-seq method with reference strains

From: IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes

    Sequence of both sides Sequence by a single termini
Strain RFLP bands PCR confirmed #IS sites False Positives False Negatives True Positives #IS sites False Positives False Negatives True Positives
Col-H37Rv 13 15 15 0 0 15 17 2 0 15
Col-UT98 11 13 10 0 3 10 13 0 0 13
Col-UT98C 11 13 10 0 3 10 13 0 0 13
Col-UT261 10 14 13 0 1 13 14 0 0 14
Arg-410 9 12 9 0 3 9 12 0 0 12
Arg-6548 8 11 8 0 3 8 11 0 0 11
Spa-C1 10 15 11 0 4 11 15 0 0 15
Total 72 93   0 17 76   2 0 93
Sensitivity 77%      82%     100%
PPV*      100%      98%
  1. *PPV: Positive Predicted value.
  2. For each reference strain the number of RFLP observed bands is reported as well as the number of IS sites identified using IS-seq by one or both termini of the insertion and the number of confirmed sites by PCR. Sensitivity and Positive Predicted value is calculated as indicated in Methods.