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Table 3 Insertions in genes important for growth or virulence

From: IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes

Locus Gene ID Description IS position within ORF (%)1 PCR verification2 Reference
Essential genes
Rv0336 - Conserved 13E12 repeat family protein 71.2 + [25]
Rv0405 pks6 Probable membrane bound polyketide synthase 26.7 + [26]
Rv1371 - Probable conserved membrane protein 17.8 + [29]
Rv1469 ctpD Probable cation transporter P-type ATPase D 2.5 + [29]
Rv1477 ripA Peptidoglycan hydrolase 72.9 + [28, 30]
Rv1753c PPE24 PPE Family protein 2.4 + [26],[25][27]
Rv1978 - Conserved hypothetical protein 41.8 + [27]
Rv2388c hemN Coproporphyrinogen III oxidase 28.7 - [29],[27]
Rv2708c - Conserved hypothetical protein 61.8 + [27]
Rv2808 - Hypothetical protein 23.6 + [29]
Rv2812 - Probable transposase 8.8 + [28, 30]
Rv2817c - Conserved hypothetical protein 71.8 + [28, 30]
Rv3018c PPE46 PPE family protein 4.7 + [28, 30]
Rv3112 moaD1 Probable molybdenum cofactor biosynthesis protein D 29.8 + [28, 30]
Rv3113 - Possible phosphatase 34.1 + [28, 30]
Rv3114 - Conserved hypothetical protein 40.7 - [29]
Rv3201c - Probable ATP-dependent DNA helicase 0.2 + [28, 30]
Rv3229c desA3 Possible linoleoyl-CoA desaturase 43.4 + [29],[27]
Rv3343c PPE54 PPE family protein 9.3 + [28, 30]
Rv3376 - Conserved hypothetical protein 60.9 + [27]
Essential genes - Less probable to affect function
Rv0001 dnaA Chromosomal replication initiation protein 95.1 + [28, 30]
Rv0755c PPE12 PPE Family protein 96.4 + [28, 30]
Rv2282c - Probable transcription regulator (LysR family) 84.9 + [27]
Rv2833c ugpB Probable Sn-glycerol-3-phosphate-binding lipoprotein 88.9 + [28, 30]
Rv2856 nicT Possible nickel-transport integral membrane protein 89.8 + [28, 30]
Rv3111 moaC1 Molybdenum cofactor biosynthesis protein C 92.8 - [28, 30]
Rv3177 - Possible peroxidase 90.4 + [28, 30]
Virulence, detoxification and adaptation category (Tuberculist)
Rv0591 mce2C MCE-family protein 56.1   
Rv1477 ripA Peptidoglycan hydrolase 72.9   
Rv1720c vapC12 Possible toxin 7.9   
Rv2494 - Conserved hypothetical protein 97.2   
Rv3176c mesT Probable epoxide hydrolase 11.8   
Rv3177 - Possible peroxidase 90.4   
Rv3473c (MT3579 bpoA Possible peroxidase 93.8   
  1. 1) Indicates the position of the insertion within the ORF as the percentage of gene upstream of the insertion.
  2. 2) Insertions verified using PCR: +, PCR positive; -, PCR negative.