Figure 5

Examples of correlations between relative abundance of gene copy and basal gene expression levels for four gene markers. Relative expression and gene copy abundance were estimated by qPCR at individual level (n = 10) for (A) wy0aba11yc21fm1.1.a.cg.2, (B) cdn20p0004b10.f.1.a.cg.2, (C) Multiple EGF-like domains 10, and (D) wy0aba18yb03fm1.1.a.cg.2. Relative expression levels were obtained according to the 2^−ΔΔCq method using the Cg-rpl40 as constitutively expressed gene. Relative abundances of gene copy were obtained with the same method but using the single copy Cg-bpi gene as reference. Grey line represents linear fit, significant correlations are indicate by P < 0.05 and r indicates the correlation coefficient.