Figure 6
From: Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes
Expression of dRif1 protein in yeast does not interfere with telomere length maintenance. Southern blot of telomeric restriction fragments from wild type (KRY-12), rif1, rif2 and rif1rif2 double mutant yeast strains transformed with either empty vector (pRS315, yEpLac181), yRif1(positive control) or dRif1 in different vectors. XhoI digests of the genomic DNA probed with dGT repeat to identify the telomeric repeat length. The median length of wild type telomeres is approximately 1.2 kb, rif1 is 2 kb, rif2 is 1.6 kb and rif1rif2 is 4 kb.