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Table 1 Breed and genotype of individuals sequenced for the presence of FGF20 c.535A > T

From: Genome-wide SNP scan of pooled DNA reveals nonsense mutation in FGF20 in the scaleless line of featherless chickens

Breed

Genotype

No. of individuals

c.496 (rs14481412)

c.535

c.552 (rs14481413)

UC Davis (low line)

sc/sc

10

CC

TT

AA

UC Davis (high line)

sc/sc

10

CC

TT

AA

Storrs, Connecticut (low line)

sc/sc

2

CC

TT

AA

Israeli experimental line

sc/sc

16

CC

TT

AA

Israeli experimental line

sc/+

3

CC

AT

AA

Israeli experimental line

sc/+

6

CC

AT

AG

New Hampshire (IFAG, Germany)

WT

6

CC

AA

AA

New Hampshire (IFAG, Germany)

WT

4

CT

AA

AG

New Hampshire, UK

WT

2

TT

AA

GG

New Hampshire, UK

WT

1

CT

AA

AG

Black Leghorn

WT

1

CC

AA

AA

Araucana

WT

1

CC

AA

AA

Old English Pheasant Fowl

WT

1

CC

AA

AA

Langshan

WT

1

CT

AA

AG

Silver Appenzeller

WT

1

CC

AA

AA

Roslin Institute experimental

WT

2

CC

AA

AA

Roslin Institute experimental

WT

1

CT

AA

AG

ISA Brown

WT

2

CC

AA

AA

Outbred, Mexico

WT

7

CC

AA

AA

Outbred, Mexico

WT

2

TT

AA

GG

Outbred, Mexico

WT

1

CT

AA

AG

Cream Legbar

WT

1

CC

AA

AA

Dorking

WT

1

CC

AA

AA

Campine

WT

1

CC

AA

AA

Naked Neck, Wernlas collection

WT

1

TT

AA

GG

Hungarian AVIANDIV Naked Neck

WT

1

TT

AA

GG

Naked Neck, English

WT

1

CC

AA

AA

Scots Grey

WT

1

TT

AA

GG

  1. Results from sequencing FGF20 exon 3 in 38 sc/sc individuals, 9 sc/+ individuals and 40 WT birds. The FGF20 c.535A > T mutation was found in all of the sc/sc and none of the WT samples sequenced. The genotype of the two flanking SNPs (rs14481412 and rs14481413) revealed that 25/40 WT individuals were homozygous for the same haplotype as scaleless, indicating that the FGF20 c.535A > T mutation does not lie within a rare haplotype.